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Uses of Brucine for Nitrate Determination from natural waters. Change of colour directly proportional of nitrate concentration.
Nitrate Test Kit based on Brucine method. Manufacturer and supplier in Mumbai, Kandla, Chennai, Vizag, Kolkata, Fujairah, Dubai, Sharjah.
Brucine Method :
Uses of Brucine for Nitrate Determination from natural waters. This method gives results in the range of 0.05 to 0.8 mg per litre nitrate nitrogen. Change of colour directly proportional of nitrate concentration.
Determination of Nitrate from water can be performed by Phenol Disulfonic Acid Method and tentatively Brucine method.
In Phenol Disulfonic Acid Method, excess chloride interference the result while Brucine method is not significantly influenced by variation of chloride concentration up to 0 to 20 grams per litre. Brucine method highly reproducible results in the range of 0.05 to 0.8 mg per litre nitrate nitrogen. The colour produced bears an essentially linear relationship to the nitrate concentration level.
Brucine methods are common for recoveries of nitrate from natural waters but brucine method is not recommended for untreated sewage from which recoveries are poor.
Test for nitrate in water :
Reagents: Brucine solution Conc. Sulphuric acid
Procedure:
. Take 1 ml of sample water in a tube with a pipette.
. Add 2 drops of Brucine solution to the tube.
. Add 2-3 drops of Conc. Sulphuric acid to the tube.
. Shake well and wait for 20 minutes.
. The solution will turn yellow in presence of nitrate. The darker the shade, the more is the concentration of nitrate in water.