COOMASIE BRILLANT BLUE R 250 FOR GEL ELECTROPHORESIS
Coomassie R-250 and G-250 dyes are two chemical forms of a disulfonated triphenylmethane
compound that is commonly used as the basis of stains for detection of proteins in gel electrophoresis and
Bradford-type assay reagents for protein quantitation.
Brilliant Blue R can be used for staining proteins on polyacrylamide electrophoresis gels. Improvement of sensitivity and background have been accomplished using Brilliant Blue dyes in a colloidal form. Other applications for brilliant Blue R include use in detection of lipids in thin layer chromatography, determination of the CMC of detergents, and detection of proteins in solution, as well as on cellulose acetate plates.
Tested for suitability on agarose gels following immunoelectrophoresis.