SILICA GEL HF 254 FOR TLC
Silica gel is by far the most widely used adsorbent and remains the dominant stationary phase for TLC.
The surface of silica gel with the highest concentration of geminal and associated silanols is favored most for the chromatography of basic compounds because these silanols are less acidic.
pH-value (10% suspension): 6.0 - 7.0
Fe (Iron): ≤ 0.02%
Calcium sulfate (CaSO₄·0.5 H₂O): 12.0 - 13.5%
Particle size: 90% <55 µm
Application test: conforms
UV absorption (Dichloro methane extract): conforms
The data to the particle size are related to the pure Silica gel before additive addition
Thin layer chromatography is done exactly as it says - using a thin, uniform layer of silica gel or alumina coated onto a piece of glass, metal or rigid plastic.
Thin layer chromatography (TLC) is an affinity-based method used to separate compounds in a mixture. ... The sample is spotted onto one end of the TLC plate and placed vertically into a closed chamber with an organic solvent (mobile phase)