Product code: RXSOL-60-6604-772
KOVACS INDOLE REAGENT
Kovacs reagent is a biochemical reagent consisting of isoamyl alcohol, para-dimethylaminobenzaldehyde (DMAB), and concentrated hydrochloric acid. ... The indole produced is indicated by formation of a red coloured ring, soluble in ether, chloroform and alcohol.
Kovacs reagent is a biochemical reagent consisting of isoamyl alcohol, para-dimethylaminobenzaldehyde (DMAB), and concentrated hydrochloric acid. ... The indole produced is indicated by formation of a red coloured ring, soluble in ether, chloroform and alcohol.
Application
It is used for the diagnostic test, to determine the ability of the organism to split tryptophan into indole and alpha-aminopropionic acid by hydrolytic activity of bacteria that express tryptophanase enzyme (3).
Dosage
Discrete bacterial colonies on solid medium. Tube method. 1% tryptophan or peptone broth. Kovác's reagent (for use with broth cultures).