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EOSINE YELLOW FOR MICROSCOPY
Eosin stain determines the percentage of living and dead cells in semen samples of all domestic mammals.
It is simple, rapid and effective.
Due to the damaged membrane, dead sperm cells absorb the stain, while living cells remain uncolored.
Eosin can be used to stain cytoplasm, red blood cells, collagen, and muscle fibers for histological examination. It is most often used as a counterstain to hematoxylin in H&E staining
It is typically pink, with different shades of pink for different types of connective tissue fibers. Eosin Y is the most commonly used form of eosin and may be used in both water and alcohol.
Eosin is anionic and acts as an acidic dye. It is negatively charged and can react with positively charged, acidophilic components in the tissue, such as amino groups in proteins in the cytoplasm. These stain pink as a result.