ORTHOPHOSPHORIC ACID FOR HPLC
orthophosphoric acid, glacial acetic acid or formic acid to make acidic and triethylamine to make it basic.
pH could be adjusted between 3-6.5 for c18 column otherwise column will get damaged.
I have different HPLC columns in the lab, which are stable on this wide pH range.
use orthophosphoric acid, glacial acetic acid or formic acid to make acidic and triethylamine to make it basic. pH could be adjusted between 3-6.5 for c18 column otherwise column will get damaged. You can adjust your pH from 1-11, if you have a HPLC column, which is pH stable.
Phosphoric acid is commonly encountered in chemical laboratories as an 85% aqueous solution, which is a colourless, odourless, and non-volatile syrupy liquid.