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INDOLE AR For estamination of DNA
Absorbance has been the method of choice for routine quantification of DNA and RNA since decades.
It is simple and convenient to use as no further sample treatment (other than DNA extraction) or reaction with other substances is required
Indole is an aromatic heterocyclic organic compound with fo rmula C8H7N. It has a bicyclic structure, consisting of a six-membered benzene ring fused to a five-membered pyrrole ring.
Thus, indole in biological samples is often estimated using the simple and rapid Kovács assay, which nonspecifically detects a variety of commonly occurring indole analogs.
Indole, a bacterial product of tryptophan degradation, has a variety of important applications in the pharmaceutical industry and is a biomarker in biological and clinical specimens. Yet, specific assays to quantitate indole are complex and require expensive equipment and a high level of training.