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WRIGHTS STAIN for microscopical staining
Wright's stain is a histology stain that facilitates the differentiation of blood cell types. It is primarily used for the initial evaluation to differentiate nuclear and/or cytoplasmic morphology of platelets, RBCs, WBCs for diagnosis, (size,form and content) and examined under microscope .
Atlas Leishman's stain is used for staining of blood and bone marrow. It is generally used to differentiate and identify leucocytes, malaria parasites, and trypanosomas (unicellar parasitic protozoa). It is based on a mixture of methylene blue and eosin.
PRINCIPLE: Wright's stain is a polychromatic stain consisting of a mixture of eosin and methylene Blue. When applied to blood cells, the dyes produce multiple colors based on the ionic charge of the stain and the various components of the cell.
Wright and Giemsa stains are used to stain peripheral blood and bone marrow smears. The components are oxidized eosin Y, methylene blue, and azure B. They stain the cytoplasm of cells an orange to pink color and nucleus a blue to purple. Wright and Giemsa stains are used to study blood cell morphology.